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mouse monoclonal anti tubulin e7  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse monoclonal anti tubulin e7
    Mouse Monoclonal Anti Tubulin E7, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+alpha+tubulin/anti-tubulin/pmc13103552-4-0-8
    Average 97 stars, based on 29 article reviews
    mouse monoclonal anti tubulin e7 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Neurogenesis in the trunk and brain of the milkweed bug Oncopeltus fasciatus: insights beyond holometabolan models.
    Article Snippet: .. After adding the primary Anti-alpha tubulin (1:50; DSHB,12G10, Uniprot ID: P41351), or anti-phosphorylated histone 3 (1:50; Sigma-Aldrich, NCBI accession no. AR TIC LE IN PR ES S NP003484.1) antibodies, the embryos were incubated for 1 hour at room temp, then overnight at 4°C. ..

    Article Title: Neurogenesis in the trunk and brain of the milkweed bug Oncopeltus fasciatus : insights beyond holometabolan models
    Article Snippet: .. After adding the primary Anti-alpha tubulin (1:50; DSHB, 12G10, Uniprot ID: P41351 ), or anti-phosphorylated histone 3 (1:50; Sigma-Aldrich, NCBI accession no. NP003484.1) antibodies, the embryos were incubated for 1 h at room temp, then overnight at 4 °C. ..

    Article Title: Heat shock factor 2 regulates oncogenic gamma-herpesvirus gene expression by remodeling the chromatin at the ORF50 and BZLF1 promoter.
    Article Snippet: .. The following primary antibodies were diluted in 5% milk-PBST and used for immunoblotting with a 1:1000 dilution for the anti-HSF2 (HPA031455, SigmaAldrich) and anti-HSF1 (SMC-118C, StressMarq Biosciences), 1:500 dilution for anti-K8.1 (sc-65446, SantaCruz); anti-KbZIP (sc-69797, SantaCruz); 1:250 anti-LANA (AB4103, abcam); anti-actin (A4700, Sigma-Aldrich); anti-alpha-tubulin (12G10, DSHB) and incubated overnight at 4°C. .. After three rounds of washing in PBST the following Horseradish peroxidaseconjugated secondary antibodies were diluted in 5% milk-PBST and used with at a 1:10000 dilution anti-rabbit (W4018, Promega), 1:5000 anti-mouse (W402B, Promega), 1:3000 anti-Rat (ab97057, abcam) and incubated at room temperature for 1 hour.

    Article Title: Heat shock factor 2 regulates oncogenic gamma-herpesvirus gene expression by remodeling the chromatin at the ORF50 and BZLF1 promoter
    Article Snippet: .. The following primary antibodies were diluted in 5% milk-PBST and used for immunoblotting with a 1:1000 dilution for the anti-HSF2 (HPA031455, Sigma-Aldrich) and anti-HSF1 (SMC-118C, StressMarq Biosciences), 1:500 dilution for anti-K8.1 (sc-65446, SantaCruz); anti-K-bZIP (sc-69797, SantaCruz); 1:250 anti-LANA (AB4103, abcam); anti-actin (A4700, Sigma-Aldrich); anti-alpha-tubulin (12G10, DSHB) and incubated overnight at 4°C. .. After three rounds of washing in PBST the following Horseradish peroxidase-conjugated secondary antibodies were diluted in 5% milk-PBST and used with at a 1:10000 dilution anti-rabbit (W4018, Promega), 1:5000 anti-mouse (W402B, Promega), 1:3000 anti-Rat (ab97057, abcam) and incubated at room temperature for 1 hour.

    Western Blot:

    Article Title: Heat shock factor 2 regulates oncogenic gamma-herpesvirus gene expression by remodeling the chromatin at the ORF50 and BZLF1 promoter.
    Article Snippet: .. The following primary antibodies were diluted in 5% milk-PBST and used for immunoblotting with a 1:1000 dilution for the anti-HSF2 (HPA031455, SigmaAldrich) and anti-HSF1 (SMC-118C, StressMarq Biosciences), 1:500 dilution for anti-K8.1 (sc-65446, SantaCruz); anti-KbZIP (sc-69797, SantaCruz); 1:250 anti-LANA (AB4103, abcam); anti-actin (A4700, Sigma-Aldrich); anti-alpha-tubulin (12G10, DSHB) and incubated overnight at 4°C. .. After three rounds of washing in PBST the following Horseradish peroxidaseconjugated secondary antibodies were diluted in 5% milk-PBST and used with at a 1:10000 dilution anti-rabbit (W4018, Promega), 1:5000 anti-mouse (W402B, Promega), 1:3000 anti-Rat (ab97057, abcam) and incubated at room temperature for 1 hour.

    Article Title: Heat shock factor 2 regulates oncogenic gamma-herpesvirus gene expression by remodeling the chromatin at the ORF50 and BZLF1 promoter
    Article Snippet: .. The following primary antibodies were diluted in 5% milk-PBST and used for immunoblotting with a 1:1000 dilution for the anti-HSF2 (HPA031455, Sigma-Aldrich) and anti-HSF1 (SMC-118C, StressMarq Biosciences), 1:500 dilution for anti-K8.1 (sc-65446, SantaCruz); anti-K-bZIP (sc-69797, SantaCruz); 1:250 anti-LANA (AB4103, abcam); anti-actin (A4700, Sigma-Aldrich); anti-alpha-tubulin (12G10, DSHB) and incubated overnight at 4°C. .. After three rounds of washing in PBST the following Horseradish peroxidase-conjugated secondary antibodies were diluted in 5% milk-PBST and used with at a 1:10000 dilution anti-rabbit (W4018, Promega), 1:5000 anti-mouse (W402B, Promega), 1:3000 anti-Rat (ab97057, abcam) and incubated at room temperature for 1 hour.

    Article Title: One-carbon metabolism enzyme Ahcy is a redox sensor that modulates gene expression to protect against light stress-induced retinal degeneration
    Article Snippet: Cells were harvested and lysed with NP-40 buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 10% glycerol) containing protease inhibitors and proteins immunoprecipitated using Anti-HA Magnetic beads (Thermo Scientific, Cat #88836). .. Western blotting was performed usinganti-HA-HRP (Roche, Cat #12013819001), anti-V5-HRP (Life Technologies, Cat #R961-25), anti-alpha-Tubulin (DSHB, Cat AA4.3). ..

    Article Title: Drosophila Ahcy is a redox sensor that modulates gene expression to protect against light stress-induced retinal degeneration.
    Article Snippet: One-carbon metabolism produces all necessary methyl units for DNA, RNA, and histone methylation reactions in the cell.. The use of these methyl units generates the by-product S -adenosylhomocysteine (SAH), a potent inhibitor of methyltransferases.. SAH is rapidly hydrolyzed by the metabolic enzyme S Adenosylhomocysteinase (Ahcy), a conserved enzyme essential for cell proliferation, chromatin accessibility, gene expression, and methylation capacity.

    other:

    Article Title: Fbxo42 promotes the degradation of Ataxin-2 granules to trigger terminal Xbp1 signaling
    Article Snippet: Input and eluted samples (in the presence/absence of DTT) were loaded onto 7.5% Mini-Protean TGX (Tris-Glycine eXtended) Precast Protein Gels (Bio-Rad) and immunoblots were performed with rat anti-HA (1:1000, 7C9, Chromotek) antibody.



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